The Dyno eCap 1 capsid outperforms all external engineered capsids and dramatically outperforms AAV2 in transducing the NHP retina following IVT delivery. Transduction of capsid variants relative to AAV2 in a library of <100 variants, measured by barcode NGS.

The Dyno eCap 1 capsid transduces the peripheral retina with high efficiency and expands into sparser patches towards the central retina. Eyes injected with Dyno eCap 1 (1.39e11 vg) packaging a multicistronic reporter expressing membrane localized GFP and nuclear mCherry.

The Dyno eCap 1 capsid transduces up to 3x better than an external engineered capsid across all layers of the retina. Histological quantification of transduction in cynomolgus retina.

Dyno eCap 1 transduces cells in all layers across the neural retina. Histological quantification of transduction in equally sized bins along the cynomolgus retina.

snRNA-seq data shows consistent transduction by the Dyno eCap 1 capsid across all retinal cell types. UMAP projection of all profiled retinal cells (right) and UMAP projection shaded by transduction events for different capsids (left).

Dyno eCap 1 delivery outperforms external capsid across cell types and regions. snRNAseq quantification of intravitreal co-injection in Cyno NHP eyes after 28 day in-life period, 2.7e11 vg total dose per eye.

The Dyno eCap 1 capsid achieves highly efficient transduction of RGCs in macular region, enabling therapeutic interventions that support survival of macular RGCs. cSLO image obtained from eGFP expression after 4 weeks postdosing with Dyno eCap 1 at 8.1e10 vg.

The Dyno eCap 1 capsid primarily transduces rod photoreceptors, enabling therapeutic interventions in inherited retinal diseases. Cell-specific co-staining of regions of transduction in the central (nonmacula) NHP retina, injected at 1.39e11 vg dose.

The Dyno eCap 1 capsid achieves highly efficient transduction of of bipolar cells, enabling optogenetic therapeutic interventions in advanced retinal disease progression. Cell-specific co-staining of regions of transduction in the central (nonmacula) NHP retina, injected at 1.39e11 vg dose.

Dyno eCap 1 capsid histology and snRNA-seq results closely match across multiple NHP experiments. Fold change in transduction of the Dyno eCap 1 capsid relative to external engineered capsid across regions of NHP retina as quantified by histology (left) and by snRNA-seq (right).